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平臺(tái)編號(hào)：bio-68925
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國(guó)際編號(hào)：CRL-10852?
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細(xì)胞信息： 0293 c18
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
293 c18
運(yùn)輸方式：凍存運(yùn)輸
年限：fetus
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
器官來(lái)源：腎臟
ATCC Number：CRL-10852?
規(guī)格：0.5mg Designations: 293 c18
Depositors: ?Stanford University
Biosafety Level:2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: kidney
Cell Type: transformed with adenovirus 5 DNA
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Age: fetus
Comments:This line is a derivative of 293 (see ATCC CRL-1573 ) the cells express EBNA1.
Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1573
年限：fetus
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
器官來(lái)源：腎臟
ATCC Number：CRL-10852?
規(guī)格：0.5mg Designations: 293 c18
Depositors: ?Stanford University
Biosafety Level:2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: kidney
Cell Type: transformed with adenovirus 5 DNA
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Age: fetus
Comments:This line is a derivative of 293 (see ATCC CRL-1573 ) the cells express EBNA1.
Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1573
