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平臺編號：bio-68924
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國際編號：CRL-1834?
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細胞信息： GK-5
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
GK-5
細胞形態(tài)：淋巴樣
細胞類型：B淋巴細胞
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：人
ATCC Number：CRL-1834?
運輸方式：凍存運輸
器官來源：外周血
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg Designations: GK-5
Depositors: ?JF Kearney
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody; against human kappa light chain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21879: Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984
22889: Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036
細胞類型：B淋巴細胞
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：人
ATCC Number：CRL-1834?
運輸方式：凍存運輸
器官來源：外周血
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg Designations: GK-5
Depositors: ?JF Kearney
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody; against human kappa light chain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
- 0.05 mM 2-mercaptoethanol
- fetal bovine serum to a final concentration of 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21879: Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984
22889: Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036
