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平臺編號：bio-68887
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國際編號：CCL-79?
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細胞信息： Y-1
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Y-1
數(shù)量：大量
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：小鼠
器官來源：其他
細胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
品系：LAF1
組織來源：cortex
ATCC Number：CCL-79?
規(guī)格：0.5mg Designations: Y-1
Depositors: ?G Sato
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:epithelial
Source: Organ: adrenal gland; tumor
Strain: LAF1
Tissue: cortex
Cellular Products:steroid hormones
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:detection of LT toxin [21447 ]
detection of cholera toxin [21556 ]
detection of verotoxin
Virus Susceptibility:Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Cytogenetic Analysis:modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected.
Gender: male
Comments:Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: At least 2 times per week (or else hormone production will decrease)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1216: Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699
21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.
21556: U.S. Food & Drug AdministrationDetection of cholera enterotoxin (CT) and cytotoxinIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 9.12-9.15, 1995
23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682
26234: Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454
26235: Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719
16173349: Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：小鼠
器官來源：其他
細胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
品系：LAF1
組織來源：cortex
ATCC Number：CCL-79?
規(guī)格：0.5mg Designations: Y-1
Depositors: ?G Sato
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:epithelial
Source: Organ: adrenal gland; tumor
Strain: LAF1
Tissue: cortex
Cellular Products:steroid hormones
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:detection of LT toxin [21447 ]
detection of cholera toxin [21556 ]
detection of verotoxin
Virus Susceptibility:Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Cytogenetic Analysis:modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected.
Gender: male
Comments:Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: At least 2 times per week (or else hormone production will decrease)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1216: Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699
21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.
21556: U.S. Food & Drug AdministrationDetection of cholera enterotoxin (CT) and cytotoxinIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 9.12-9.15, 1995
23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682
26234: Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454
26235: Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719
16173349: Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570
