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Gr431
    Gr431
  • 平臺編號:bio-68882
  • 國際編號:HB-8575?
  • 細(xì)胞信息: Gr431
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

數(shù)量:大量
運輸方式:凍存運輸
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM
ATCC Number:HB-8575?
生長狀態(tài):懸浮生長
是否是腫瘤細(xì)胞:0
物種來源:人
規(guī)格:0.5ml Designations: Gr431
Depositors: ?Sloan-Kettering Institute for Cancer Research
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism:human (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Lymphocytes were obtained from the regional lymph nodes of a patient who had breast cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 90%; DMSO, 10%
References: 3897: Thomson TM, et al. Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987

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