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2D-7
    2D-7
  • 平臺(tái)編號(hào):bio-68877
  • 國(guó)際編號(hào):HB-9667?
  • 細(xì)胞信息: 2D-7
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
免疫類型:IgG1
數(shù)量:大量
ATCC Number:HB-9667?
規(guī)格:0.5mg Designations: 2D-7
Depositors: ?Johns Hopkins University
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human CENP-B peptide.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a major epitope on human CENP-B.
The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22021: Earnshaw W, et al. Cloned human centromere autoantigen. US Patent 5,196,307 dated Mar 23 1993

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