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平臺編號：bio-68871
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國際編號：CRL-1742?
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細(xì)胞信息： Y13-259
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Y13-259
生長狀態(tài)：懸浮生長
數(shù)量：大量
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
ATCC Number：CRL-1742?
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
規(guī)格：0.5mg Designations: Y13-259
Depositors: ?JR Stephenson, ME Furth
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.
Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.
The antibody reacts with both the Harvey (Ha-ras) and Kirsten (Ki-ras) p21 proteins.
The antibody also precipitates an endogenous p21 from a variety of rodent, carnivore and primate cell lines.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982. PubMed: 6287003
數(shù)量：大量
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：褐鼠
ATCC Number：CRL-1742?
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
規(guī)格：0.5mg Designations: Y13-259
Depositors: ?JR Stephenson, ME Furth
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.
Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.
The antibody reacts with both the Harvey (Ha-ras) and Kirsten (Ki-ras) p21 proteins.
The antibody also precipitates an endogenous p21 from a variety of rodent, carnivore and primate cell lines.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982. PubMed: 6287003
