器官來源:皮膚
細(xì)胞形態(tài):成纖維樣
運輸方式:凍存運輸
是否是腫瘤細(xì)胞:0
物種來源:人
生長狀態(tài):貼壁生長
年限:48 years
ATCC Number:CRL-1672?
相關(guān)疾?。杭拥录{綜合征
數(shù)量:大量
規(guī)格:0.5mg Designations: GS-109-IV-8
Depositors: ?EJ Gardner
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: Gardner's syndrome
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments:The tissue donor suffered from Gardner's syndrome.
The cells are said to exist in an initiated state as opposed to a normal or transformed state.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.