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2D7F10
    2D7F10
  • 平臺(tái)編號(hào):bio-68847
  • 國際編號(hào):CRL-2025?
  • 細(xì)胞信息: 2D7F10
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

是否是腫瘤細(xì)胞:0
物種來源:小鼠
生長狀態(tài):懸浮生長
數(shù)量:大量
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類型:IgA
ATCC Number:CRL-2025?
規(guī)格:0.5ml Designations: 2D7F10
Depositors: ?KW Ruby
Isotype: IgA
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Leptospira pomona type kennewicki
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to agglutinates L. interogans serovar pomona type kennewicki, and binds to type kennewicki antigen in ELISA.
It can be used to identify type kennewicki in veterinary biologicals.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 24407: . . Proceedings of the 94th Annual Meeting of the United States Animal Health Association, Denver, CO, : 290-294, 1990.

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