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平臺(tái)編號(hào)：bio-68844
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國(guó)際編號(hào)：CRL-1689?
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細(xì)胞信息： 2D12
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
2D12
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a; kappa light chain
ATCC Number：CRL-1689?
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations: 2D12
Depositors: ?JJ Schlesinger
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d
Comments:Mice were immunized with the 17D strain of yellow fever virus.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody does not cross-react with other flaviviruses.
Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium containing non-essential amino acids, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, and 0.02 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 1014: Schlesinger JJ, et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129
1015: Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a; kappa light chain
ATCC Number：CRL-1689?
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations: 2D12
Depositors: ?JJ Schlesinger
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d
Comments:Mice were immunized with the 17D strain of yellow fever virus.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody does not cross-react with other flaviviruses.
Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium containing non-essential amino acids, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, and 0.02 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 1014: Schlesinger JJ, et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129
1015: Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738
