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平臺編號：bio-68831
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國際編號：HB-8508?
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細(xì)胞信息： 225
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
225
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 , IgG1
生長狀態(tài)：懸浮生長
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：HB-8508?
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: 225
Depositors: ?Regents of the University of California
Isotype: IgG1 , IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor and inhibits the growth of A-431 and T222 tumors in nude mice (although not as strongly as 528 (see ATCC HB-8509 ).
The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts, and it blocks EGF stimulated phosphorylation of membrane proteins.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; horse serum, 15%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2040
References: 21948: Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990
92782: Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961
免疫類型：IgG1 , IgG1
生長狀態(tài)：懸浮生長
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：HB-8508?
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: 225
Depositors: ?Regents of the University of California
Isotype: IgG1 , IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor and inhibits the growth of A-431 and T222 tumors in nude mice (although not as strongly as 528 (see ATCC HB-8509 ).
The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts, and it blocks EGF stimulated phosphorylation of membrane proteins.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; horse serum, 15%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2040
References: 21948: Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990
92782: Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961
