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運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：其他
數(shù)量：大量
年限：fetus
生長狀態(tài)：貼壁生長
ATCC Number：CCL-193?
器官來源：肺
相關(guān)疾病：正常
規(guī)格：0.5ml                                                                                                                                                                                                                                                                                                                                                          Designations: R9ab
         Depositors:                  ?EM Earley
         Biosafety Level:1
         Shipped:        frozen
         Medium & Serum: See Propagation          Growth        Properties:adherent
         Organism:          Oryctolagus cuniculus
         Morphology:fibroblast
         

         Source:          Organ: lung
         Disease: normal
         Cell Type: fibroblast
         Permits/Forms:In addition to the MTA          mentioned above, other ATCC            and/or regulatory permits  may be required for the          transfer of this ATCC material. Anyone purchasing ATCC          material is ultimately responsible for obtaining the permits.          Please click here  for information regarding          the specific requirements for shipment to your location.
             Cytogenetic        Analysis:normal; diploid; stable
         Age:        fetus
         Gender:        female
         Comments:R9ab was derived from explants of          biopsy material. Subsequent cell outgrowths were trypsinized          and the resulting cell suspension was used to initiate the          line.
The donor indicated that the life expectancy of          this line is at least 60 passages.
         Propagation:                   ATCC complete growth medium:  The base          medium for this cell line is ATCC-formulated Eagle's Minimum          Essential Medium, Catalog No. 30-2003.  To make the complete          growth medium, add the following components to the base          medium:  fetal bovine serum to a final concentration of 10%.
         Temperature: 37.0°C
         Subculturing:                  Subcultivation Ratio: A subcultivation ratio          of 1:2 to 1:3 is recommended
         Medium Renewal: Every 2 to 3 days
         Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA          solution. Remove the solution and add an additional 1 to 2 ml          of trypsin-EDTA solution. Allow the flask to sit at room          temperature (or at 37C) until the cells detach.
Add          fresh culture medium, aspirate and dispense into new culture flasks.
         Preservation:        culture medium 95%; DMSO, 5%
         Related        Products:Recommended medium (without the          additional supplements or serum described under ATCC          Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
         References:           32878: Castagliuolo I, et al.          Saccharomyces boulardii protease inhibits Clostridium          difficile toxin A effects in the rat ileum.  Infect. Immun.          64: 5225-5232, 1996. PubMed: 8945570