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S194/5.XXO-1
    S194/5.XXO-1
  • 平臺編號:bio-68812
  • 國際編號:TIB-19?
  • 細(xì)胞信息: S194/5.XXO-1
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
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  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

品系:BALB/c
ATCC Number:TIB-19?
運(yùn)輸方式:凍存運(yùn)輸
相關(guān)疾?。憾喟l(fā)性骨髓瘤
數(shù)量:大量
生長狀態(tài):懸浮生長
細(xì)胞形態(tài):淋巴樣
免疫類型:IgA (not secreted); kappa light chain (not secreted)
細(xì)胞類型:B淋巴細(xì)胞
是否是腫瘤細(xì)胞:0
物種來源:小鼠
規(guī)格:0.5ml Designations: S194/5.XXO-1
Depositors: ?R Hyman, P Ralph, S Sarkar
Isotype: IgA (not secreted); kappa light chain (not secreted)
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; not secreted
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d; PC.1
Comments:This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Both heavy and light chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22522: Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881
33006: Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789

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