細(xì)胞形態(tài):淋巴樣
免疫類型:IgG
ATCC Number:CRL-1989?
數(shù)量:大量
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來(lái)源:倉(cāng)鼠
規(guī)格:0.5ml Designations: UC7-13D5
Depositors: ?JA Bluestone
Isotype: IgG
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for all murine TCR gamma/delta heterodimers.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: 90% complete growth medium, 10% DMSO.
References: 22362: Bluestone JA, et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760