細胞類型:T淋巴細胞
是否是腫瘤細胞:0
物種來源:小鼠
運輸方式:凍存運輸
數(shù)量:大量
ATCC Number:TIB-29?
相關疾?。毫馨土?br />
生長狀態(tài):懸浮生長
品系:BALB/c
細胞形態(tài):淋巴樣
規(guī)格:0.5ml Designations: S49.1H.1AG.6/2
Depositors: ?M Cohn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
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Antigen Expression:H-2d; Thy-1.2; TL
Comments:The cells are resistant to 0.01 mM cortisol and 1 mM 8-azaguanine.
The line was derived from S49.1 (ATCC TIB-28 ) by selection for resistance to cortisol and 8-azaguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and maintain between 3 X 10 exp5 and 7 X 10 exp5 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22241: Schiller JG, et al. Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230