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35.1
    35.1
  • 平臺(tái)編號(hào):bio-68712
  • 國(guó)際編號(hào):HB-222?
  • 細(xì)胞信息: 35.1
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

數(shù)量:大量
ATCC Number:HB-222?
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
免疫類(lèi)型:IgG2a
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
規(guī)格:10mg Designations: 35.1
Depositors: ?PJ Martin
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human CD2
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the human T cell surface protein Tp50 (CD2).
The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
References: 1792: Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907

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