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平臺編號：bio-68711
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國際編號：TIB-227?
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細(xì)胞信息： 33D1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
33D1
運輸方式：凍存運輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
數(shù)量：大量
ATCC Number：TIB-227?
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：懸浮生長
規(guī)格：100ug Designations: 33D1
Depositors: ?RM Steinman
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse spleen and lymph node dendritic cells.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antigen recognized by the 33D1 antibody appears to be present only on dendritic cells (10000 to 15000 sites/cell).
The antigen does not appear to be immune response associated antigen or Ia antigen.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1663: Nussenzweig MC, et al. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161-165, 1982. PubMed: 6948298
1664: Steinman RM, et al. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613-627, 1983. PubMed: 6185614
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
數(shù)量：大量
ATCC Number：TIB-227?
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：懸浮生長
規(guī)格：100ug Designations: 33D1
Depositors: ?RM Steinman
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse spleen and lymph node dendritic cells.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antigen recognized by the 33D1 antibody appears to be present only on dendritic cells (10000 to 15000 sites/cell).
The antigen does not appear to be immune response associated antigen or Ia antigen.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1663: Nussenzweig MC, et al. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161-165, 1982. PubMed: 6948298
1664: Steinman RM, et al. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613-627, 1983. PubMed: 6185614
