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3Pt12B8
    3Pt12B8
  • 平臺編號:bio-68701
  • 國際編號:HB-8136?
  • 細胞信息: 3Pt12B8
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

生長狀態(tài):懸浮生長
ATCC Number:HB-8136?
是否是腫瘤細胞:0
物種來源:小鼠
數(shù)量:大量
細胞形態(tài):淋巴樣
免疫類型:IgM
運輸方式:凍存運輸
規(guī)格:10mg Designations: 3Pt12B8
Depositors: ?Sidney Farber Cancer Institute
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with human T chronic lymphocytic leukemia cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody has been used to prevent graft versus host disease (GVHD).
Propagation: ATCC complete growth medium: Modified Dulbecco's medium; horse serum, 10%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3512: Reinherz EL, Schlossman SF. Monoclonal antibody. US Patent 4,443,427 dated Apr 17 1984

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