生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
品系:C57BL/6N
ATCC Number:TIB-41?
相關(guān)疾病:淋巴瘤
細(xì)胞類型:T淋巴細(xì)胞
是否是腫瘤細(xì)胞:0
物種來源:小鼠
細(xì)胞形態(tài):淋巴樣
運(yùn)輸方式:凍存運(yùn)輸
數(shù)量:大量
規(guī)格:10ml Designations: EL4.BU.1.OUAr.1.1
Depositors: ?R Hyman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: T lymphocyte;
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Antigen Expression:H-2b; Thy-1.2
Comments:This line was derived from the EL4 cell line (see ATCC TIB-39 ).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22247: Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.