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平臺編號：bio-68678
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國際編號：CRL-2296?
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細(xì)胞信息： SJD.1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SJD.1
ATCC Number：CRL-2296?
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：斑馬魚
器官來源：其他
品系：SJD
年限：adult
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100ml Designations: SJD.1
Depositors: ?BH Paw, LI Zon
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Danio rerio
Morphology:fibroblast
Source: Organ: caudal fin
Strain: SJD
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Comments:SJD.1 is a fibroblast cell line derived from amputated caudal fins of an adult zebrafish, strain SJD.
This strain of zebrafish is used for genetic mapping.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 28.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with trypsin (0.25%)-EDTA (0.03%)-polyvinylpyrrolidone (0.5%) solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 43315: Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：斑馬魚
器官來源：其他
品系：SJD
年限：adult
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100ml Designations: SJD.1
Depositors: ?BH Paw, LI Zon
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Danio rerio
Morphology:fibroblast
Source: Organ: caudal fin
Strain: SJD
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Comments:SJD.1 is a fibroblast cell line derived from amputated caudal fins of an adult zebrafish, strain SJD.
This strain of zebrafish is used for genetic mapping.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 28.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with trypsin (0.25%)-EDTA (0.03%)-polyvinylpyrrolidone (0.5%) solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 43315: Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354
