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平臺(tái)編號(hào)：bio-68635
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國際編號(hào)：CCL-156?
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細(xì)胞信息： RPMI 1788
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
RPMI 1788
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
器官來源：外周血
年限：33 years
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM; lambda light chain
ATCC Number：CCL-156?
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：1mg Designations: RPMI 1788
Depositors: ?GE Moore
Isotype: IgM; lambda light chain
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA A2, Aw33, B7, B14
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Cytogenetic Analysis:human male; hypodiploid; stable
Isoenzymes: G6PD, B
Age: 33 years
Gender: male
Ethnicity: Caucasian
Comments:The cells are EBNA positive.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium.Cultures are established at 3 X 10 exp5 viable cells/ml.
References: 22796: Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523
22918: Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807
23213: Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 5353241
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
器官來源：外周血
年限：33 years
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM; lambda light chain
ATCC Number：CCL-156?
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：1mg Designations: RPMI 1788
Depositors: ?GE Moore
Isotype: IgM; lambda light chain
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA A2, Aw33, B7, B14
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Cytogenetic Analysis:human male; hypodiploid; stable
Isoenzymes: G6PD, B
Age: 33 years
Gender: male
Ethnicity: Caucasian
Comments:The cells are EBNA positive.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium.Cultures are established at 3 X 10 exp5 viable cells/ml.
References: 22796: Aggarwal BB, et al. Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. J. Biol. Chem. 259: 686-691, 1984. PubMed: 6608523
22918: Gray PW, et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312: 721-724, 1984. PubMed: 6334807
23213: Huang CC, Moore GE. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 5353241
