ATCC Number：CRL-2139?
相關(guān)疾?。浩渌膊?br />
細胞形態(tài)：上皮樣
是否是腫瘤細胞：0
物種來源：人
器官來源：其他
數(shù)量：大量
生長狀態(tài)：貼壁生長
年限：17 years
運輸方式：凍存運輸
規(guī)格：1mg Designations: SK-PN-DW
Depositors: ?C Helson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: retroperitoneal embryonal tumor
Disease: malignant primitive neuroectodermal tumor
Cellular Products:cholecystokinin (CCK)
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Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16
Cytogenetic Analysis:aneuploid; modal number = 40 to 41; monosomy 13 was found in 100% of the cells; iso(17q) and monosomy 9, 10, 11, and 18 were found in greater than 20% of cells analyzed., In a large proportion of the cells, the double minutes per cell were too numerous to count.
Age: 17 years
Gender: male
Ethnicity: Caucasian
Comments:SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22258: Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811
22763: Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982
