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平臺編號：bio-68622
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國際編號：CRL-1568?
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細(xì)胞信息： 3197-3 (3T3-MSV)
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
3197-3 (3T3-MSV)
運輸方式：凍存運輸
器官來源：胚胎
生長狀態(tài)：貼壁生長
細(xì)胞形態(tài)：成纖維樣
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：小鼠
數(shù)量：大量
年限：embryo
ATCC Number：CRL-1568?
規(guī)格：1mg Designations: 3197-3 (3T3-MSV)
Depositors: ?JE DeLarco
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Cell Type: fibroblast
Cellular Products:The cells release a growth factor in serum free medium.
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
The cells have a tendency to slough off the flask surface.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 95%; bovine calf serum, 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days.
References: 23261: de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512
26089: Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. PubMed: 4319233
器官來源：胚胎
生長狀態(tài)：貼壁生長
細(xì)胞形態(tài)：成纖維樣
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：小鼠
數(shù)量：大量
年限：embryo
ATCC Number：CRL-1568?
規(guī)格：1mg Designations: 3197-3 (3T3-MSV)
Depositors: ?JE DeLarco
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Cell Type: fibroblast
Cellular Products:The cells release a growth factor in serum free medium.
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
The cells have a tendency to slough off the flask surface.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 95%; bovine calf serum, 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days.
References: 23261: de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512
26089: Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. PubMed: 4319233
