![4B2-6C3-1C9 [1C9]](/static/images/cells-no_picture.jpg)
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平臺(tái)編號(hào)：bio-68598
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國(guó)際編號(hào)：HB-12466?
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細(xì)胞信息： 4B2-6C3-1C9 [1C9]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
4B2-6C3-1C9 [1C9]
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
數(shù)量：大量
ATCC Number：HB-12466?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgG1
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：1ml Designations: 4B2-6C3-1C9 [1C9]
Depositors: ?University of Massachusetts Medical Center
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Norwalk Virus
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with stool-derived Norwalk virus.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody is directed against an epitope of the 58 kDa antigen of Norwalk Virus. [56174 ]
The antibody specifically binds to Norwalk virus particles found in human stool specimens from naturally occurring Norwalk virus outbreaks. [56174 ]
The antibody reacts with the prototype 8FIIa strain of Norwalk virus in an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. [56263 ]
No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses. [56263 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 56174: Herrmann JE, Blacklow NR. Monoclonal antibodies for detecting Norwalk virus. US Patent 5,861,241 dated Jan 19 1999
56263: Herrmann JE, et al. Monoclonal antibodies for detection of Norwalk virus antigen in stools. J. Clin. Microbiol. 33: 2511-2513, 1995. PubMed: 7494063
56265: Brinker JP, et al. Detection of Norwalk virus and other genogroup 1 human caliciviruses by a monoclonal antibody, recombinant-antigen-based immunoglobulin M capture enzyme immunoassay. J. Clin. Microbiol. 36: 1064-1069, 1998. PubMed: 9542938
物種來(lái)源：小鼠
數(shù)量：大量
ATCC Number：HB-12466?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgG1
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：1ml Designations: 4B2-6C3-1C9 [1C9]
Depositors: ?University of Massachusetts Medical Center
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Norwalk Virus
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with stool-derived Norwalk virus.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody is directed against an epitope of the 58 kDa antigen of Norwalk Virus. [56174 ]
The antibody specifically binds to Norwalk virus particles found in human stool specimens from naturally occurring Norwalk virus outbreaks. [56174 ]
The antibody reacts with the prototype 8FIIa strain of Norwalk virus in an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. [56263 ]
No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses. [56263 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 56174: Herrmann JE, Blacklow NR. Monoclonal antibodies for detecting Norwalk virus. US Patent 5,861,241 dated Jan 19 1999
56263: Herrmann JE, et al. Monoclonal antibodies for detection of Norwalk virus antigen in stools. J. Clin. Microbiol. 33: 2511-2513, 1995. PubMed: 7494063
56265: Brinker JP, et al. Detection of Norwalk virus and other genogroup 1 human caliciviruses by a monoclonal antibody, recombinant-antigen-based immunoglobulin M capture enzyme immunoassay. J. Clin. Microbiol. 36: 1064-1069, 1998. PubMed: 9542938
