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平臺編號：bio-68593
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國際編號：ATCC CRL-2390
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細胞信息： FL83B
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
FL83B
運輸方式：凍存運輸
器官來源：肝
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-2390?
相關(guān)疾?。赫?br /> 細胞形態(tài)：上皮樣
年限：15 to 17 days gestation
生長狀態(tài)：貼壁生長
數(shù)量：大量
規(guī)格：48T Designations: FL83B
Depositors: ?EH Leiter
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:epithelial
Source: Organ: liver
Disease: normal
Cell Type: hepatocyte;
Cellular Products:cholesterol; glycogen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Bar Harbor Maine, United States
Isolation date: 1969
Cytogenetic Analysis:The cells are aneuploid. Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Age: 15 to 17 days gestation
Comments:The cells are reported to store glycogen and actively synthesize cholesterol. They have also been reported to be able to produce serum proteins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 38860: Breslow JL, et al. Characterization of the mouse liver cell line FL83B. Exp. Cell Res. 78: 441-453, 1973. PubMed: 4572697
器官來源：肝
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-2390?
相關(guān)疾?。赫?br /> 細胞形態(tài)：上皮樣
年限：15 to 17 days gestation
生長狀態(tài)：貼壁生長
數(shù)量：大量
規(guī)格：48T Designations: FL83B
Depositors: ?EH Leiter
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:epithelial
Source: Organ: liver
Disease: normal
Cell Type: hepatocyte;
Cellular Products:cholesterol; glycogen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Bar Harbor Maine, United States
Isolation date: 1969
Cytogenetic Analysis:The cells are aneuploid. Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Age: 15 to 17 days gestation
Comments:The cells are reported to store glycogen and actively synthesize cholesterol. They have also been reported to be able to produce serum proteins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 38860: Breslow JL, et al. Characterization of the mouse liver cell line FL83B. Exp. Cell Res. 78: 441-453, 1973. PubMed: 4572697
