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ATCC Number：CRL-2148?
相關疾病：纖維肉瘤
是否是腫瘤細胞：0
物種來源：小鼠
細胞形態(tài)：成纖維樣
生長狀態(tài)：混合型生長
運輸方式：凍存運輸
品系：BALB/c
數(shù)量：大量
規(guī)格：0.2ml Designations: WEHI-13VAR
Depositors: ?JA Armstrong
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed, adherent and suspension
Organism: Mus musculus
Morphology:fibroblast


Source: Disease: fibrosarcoma
Strain: BALB/c
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:WEHI-13VAR, is a variant of WEHI 164 clone 13 which has lost its sensitivity to Tumor Necrosis Factor (TNF) in the absence of actinomycin D.
The WEHI-13VAR cell line was found to maintain its high sensitivity to TNF when the assay was performed in the presence of 500 ng/ml actinomycin D.
This line provides a stable and highly sensitive bioassay system to detect and measure mouse and human natural and recombinant tumor necrosis factors (TNF alpha and lymphotoxin.
It is more sensitive to TNF alpha and lymphotoxin than L929 (ATCC CCL-1 ) or WEHI 164 (ATCC CRL-1751 ).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove culture medium to a centrifuge tube.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 xg for 5 to10 minutes.
6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
7. Place culture vessels in incubators at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22472: Espevik T, Nissen-Meyer J. A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95: 99-105, 1986. PubMed: 3782828
22786: Abu-Khabar KS, et al. Type I interferons (IFN-alpha and -beta) suppress cytotoxin (tumor necrosis factor-alpha and lymphotoxin) production by mitogen-stimulated human peripheral blood mononuclear cell. J. Leukocyte Biol. 52: 165-173, 1992. PubMed: 1506772
24378: Khabar KS, et al. WEHI-13VAR: a stable and sensitive variant of WEHI 164 clone 13 fibrosarcoma for tumor necrosis factor bioassay. Immunol. Lett. 46: 107-110, 1995. PubMed: 7590904