![MYC 1-9E10.2 [9E10]](/static/images/cells-no_picture.jpg)
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平臺編號：bio-68574
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國際編號：CRL-1729
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細胞信息： MYC 1-9E10.2 [9E10]
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
MYC 1-9E10.2 [9E10]
ATCC Number：CRL-1729?
運輸方式：凍存運輸
生長狀態(tài)：懸浮生長
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
細胞形態(tài)：淋巴樣
免疫類型：IgG1
規(guī)格：0.2ml Designations: MYC 1-9E10.2 [9E10]
Depositors: ?J Bishop, GI Evan
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human myc (c-myc) protein
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Tested and found negative for ectromelia virus (mousepox).
Comments:Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1189: Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782
29119: Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077
33026: Cheng M, et al. ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539
運輸方式：凍存運輸
生長狀態(tài)：懸浮生長
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
細胞形態(tài)：淋巴樣
免疫類型：IgG1
規(guī)格：0.2ml Designations: MYC 1-9E10.2 [9E10]
Depositors: ?J Bishop, GI Evan
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human myc (c-myc) protein
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Tested and found negative for ectromelia virus (mousepox).
Comments:Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1189: Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782
29119: Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077
33026: Cheng M, et al. ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539
