ATCC Number：CRL-1722?
運輸方式：凍存運輸
相關疾?。毫馨土?br />
數(shù)量：大量
品系：DBA/2
器官來源：胸腺
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
規(guī)格：0.5mg Designations: L5178-R (LY-R)
Depositors: ?JZ Beer
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Organ: thymus
Strain: DBA/2
Disease: lymphoma
Cell Type: chemically induced
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Applications:This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723 ) was derived from this line.
Tested and found negative for ectromelia virus.
Tumorigenic:Yes
Comments:This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
After 6 months to 3 years in culture, the line converts to an X-ray sensitive state.
The X-ray sensitive L5178-S line (ATCC CRL-1723 ) was derived from this line.
Tested and found negative for ectromelia virus.
Propagation: ATCC complete growth medium: Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing: Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and maintain between 3 X 10 exp4 and 7 X 10 exp5 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 1164: Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899
1166: Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332
58059: Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312
