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平臺編號：bio-68568
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國際編號：CCL-95.1?
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細胞信息： WI-26 VA4
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
WI-26 VA4
數(shù)量：大量
年限：3 month gestation
ATCC Number：CCL-95.1?
生長狀態(tài)：貼壁生長
細胞形態(tài)：上皮樣
器官來源：肺
細胞類型：SV40轉(zhuǎn)化細胞
是否是腫瘤細胞：0
物種來源：人
運輸方式：凍存運輸
規(guī)格：0.2ml Designations: WI-26 VA4
Depositors: ?AJ Girardi
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial-like
Source: Organ: lung
Cell Type: SV40 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 9,11
D5S818: 11,12
D7S820: 10,13
THO1: 6,7
TPOX: 10,11
vWA: 17,20
Cytogenetic Analysis:Chromosome Frequency Distribution 49 Cells: 2n = 46Karyotype unstable within triploid stemline range of 67 to 71. Ninety-six percent of the cells have a large chromosome with a submedian centromere. Approximately 52% of the cells have one or more secondary constrictions; 42% of the cells examined had breaks.
Isoenzymes: G6PD, B
Age: 3 month gestation
Gender: male
Ethnicity: Caucasian
Comments:WI-26 VA4 is an SV40 virus transformed derivative of WI-26 a human diploid fibroblast cell line from embryonic lung tissue. After initial infection and crisis-phase of SV40-transformation, the cells underwent a change in morphology. The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22194: Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659
22197: Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085
22401: Girardi AJ, et al. SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell. Comp. Physiol. 65: 69-84, 1965.
26230: Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.
年限：3 month gestation
ATCC Number：CCL-95.1?
生長狀態(tài)：貼壁生長
細胞形態(tài)：上皮樣
器官來源：肺
細胞類型：SV40轉(zhuǎn)化細胞
是否是腫瘤細胞：0
物種來源：人
運輸方式：凍存運輸
規(guī)格：0.2ml Designations: WI-26 VA4
Depositors: ?AJ Girardi
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial-like
Source: Organ: lung
Cell Type: SV40 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 9,11
D5S818: 11,12
D7S820: 10,13
THO1: 6,7
TPOX: 10,11
vWA: 17,20
Cytogenetic Analysis:Chromosome Frequency Distribution 49 Cells: 2n = 46Karyotype unstable within triploid stemline range of 67 to 71. Ninety-six percent of the cells have a large chromosome with a submedian centromere. Approximately 52% of the cells have one or more secondary constrictions; 42% of the cells examined had breaks.
Isoenzymes: G6PD, B
Age: 3 month gestation
Gender: male
Ethnicity: Caucasian
Comments:WI-26 VA4 is an SV40 virus transformed derivative of WI-26 a human diploid fibroblast cell line from embryonic lung tissue. After initial infection and crisis-phase of SV40-transformation, the cells underwent a change in morphology. The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22194: Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659
22197: Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085
22401: Girardi AJ, et al. SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell. Comp. Physiol. 65: 69-84, 1965.
26230: Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.
