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平臺編號：bio-68564
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國際編號：HTB-102?
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細(xì)胞信息： Malme-3
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Malme-3
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
器官來源：皮膚
年限：43 years adult
ATCC Number：HTB-102?
相關(guān)疾病：正常
細(xì)胞類型：成纖維細(xì)胞
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：貼壁生長
規(guī)格：0.1ml Designations: Malme-3
Depositors: ?J Fogh
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Antigen Expression:HLA A2, Aw30, B13, B40(+/-), DRw7
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,13
D16S539: 9,12
D5S818: 11,13
D7S820: 9,12
THO1: 7,8
TPOX: 8,9
vWA: 15,16
Cytogenetic Analysis:This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 43 years adult
Gender: male
Ethnicity: Caucasian
Comments:This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64 ) was derived. Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Interval: Subculture every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
derived from same individual:ATCC HTB-64
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
數(shù)量：大量
器官來源：皮膚
年限：43 years adult
ATCC Number：HTB-102?
相關(guān)疾病：正常
細(xì)胞類型：成纖維細(xì)胞
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：貼壁生長
規(guī)格：0.1ml Designations: Malme-3
Depositors: ?J Fogh
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Antigen Expression:HLA A2, Aw30, B13, B40(+/-), DRw7
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,13
D16S539: 9,12
D5S818: 11,13
D7S820: 9,12
THO1: 7,8
TPOX: 8,9
vWA: 15,16
Cytogenetic Analysis:This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 43 years adult
Gender: male
Ethnicity: Caucasian
Comments:This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64 ) was derived. Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37C.
Interval: Subculture every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
derived from same individual:ATCC HTB-64
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
