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平臺(tái)編號(hào)：bio-68550
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國(guó)際編號(hào)：CRL-9518
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細(xì)胞信息： L5178Y TK+/- clone (3.7.2C) [TK+/- (clone 3.7.2C)]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Mus musculus, mouse
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-9518
相關(guān)疾?。毫馨土?br />
品系：DBA/2
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: L5178Y TK+/- clone (3.7.2C) [TK+/- (clone 3.7.2C)]
Depositors: ?Mobil Oil Corporation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: DBA/2
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This line is a derivative of L5178Y-S (ATCC CRL-1723 ).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1% Pluronic, 90%; fetal bovine serum, 10%
Temperature: 37.0℃
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21875: . . Chem. Mutagens 3: pp. 79-103, 1973.
