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是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：HB-2?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：mouse IgG1 kappa
年限：adult
生長狀態(tài)：懸浮生長
品系：BALB/c
運輸方式：凍存運輸
器官來源：脾
規(guī)格：0.5mg Designations: T3-3A1 [3A1]
Depositors: ?A Fauci, BF Haynes
Isotype: mouse IgG1 kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphocyte; somatic cell hybrid
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Comments:The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia.
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22 .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0℃
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) cells/ml
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 908: Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538
916: Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676
949: Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457
32301: Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803