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平臺(tái)編號(hào)：bio-68542
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國(guó)際編號(hào)：CRL-1975?
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細(xì)胞信息： 145-2C11
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
145-2C11
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-1975?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG , IgG
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：倉(cāng)鼠
規(guī)格：0.5mg Designations: 145-2C11
Depositors: ?JA Bluestone
Isotype: IgG , IgG
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse CD3
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.
The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
Comments:Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 23298: Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524
28992: Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079
ATCC Number：CRL-1975?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG , IgG
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：倉(cāng)鼠
規(guī)格：0.5mg Designations: 145-2C11
Depositors: ?JA Bluestone
Isotype: IgG , IgG
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse CD3
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.
The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
Comments:Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 23298: Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524
28992: Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079
