5,378,056
品系：NIH/Swiss
細胞形態(tài)：成纖維樣
數量：大量
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
年限：embryo
器官來源：胚胎
細胞類型：成纖維細胞
ATCC Number：CRL-9642?
是否是腫瘤細胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations: GP+E-86
Depositors: ?Trustees of Columbia University
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Age: embryo
Comments:This line is capable of packaging nucleic acids containing a psi packaging sequence into recombinant ecotropic retrovirus genomes.
It can be used to produce retroviral vectors for delivery of foreign genes into susceptible eukaryotic cells.
The line was established by electroporation of two plasmids into NIH 3T3 cells.
One plasmid contained the gag and pol regions of Moloney murine leukemia virus (Mo-MuLV), the other contained the env region of Mo-MuLV.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.015 mg/ml hypoxanthine, 0.25 mg/ml xanthine, and 0.025 mg/ml mycophenolic acid; 90% calf serum, 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
References: 22050: Bank A, et al. Retroviral packaging cell lines and process of using same. US Patent 5,278,056 dated Jan 11 1994
