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平臺(tái)編號(hào)：bio-68527
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國(guó)際編號(hào)：TIB-76?
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細(xì)胞信息： BNL 1NG A.2
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
BNL 1NG A.2
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：BALB/c
數(shù)量：大量
年限：embryo
器官來(lái)源：肝
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：TIB-76?
相關(guān)疾病：正常
規(guī)格：0.1ml Designations: BNL 1NG A.2
Depositors: ?P Patek, J Collins, M Cohn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Organ: liver
Strain: BALB/c
Disease: normal
Cell Type: chemically transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Age: embryo
Comments:This line was derived from BNL CL.2 (ATCC TIB-73 ) by transformation with nitrosoguanidine.
The cells will grow in 0.3% agarose.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：BALB/c
數(shù)量：大量
年限：embryo
器官來(lái)源：肝
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：TIB-76?
相關(guān)疾病：正常
規(guī)格：0.1ml Designations: BNL 1NG A.2
Depositors: ?P Patek, J Collins, M Cohn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Organ: liver
Strain: BALB/c
Disease: normal
Cell Type: chemically transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Age: embryo
Comments:This line was derived from BNL CL.2 (ATCC TIB-73 ) by transformation with nitrosoguanidine.
The cells will grow in 0.3% agarose.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934
