是否是腫瘤細胞:0
物種來源:小鼠
數(shù)量:大量
ATCC Number:TIB-108?
生長狀態(tài):懸浮生長
年限:adult
運輸方式:凍存運輸
器官來源:脾
細胞形態(tài):淋巴樣
免疫類型:IgM lambda
品系:BALB/c
規(guī)格:1mg Designations: N-S.2.1
Depositors: ?WC Raschke
Isotype: IgM lambda
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: lymphocyte B lymphocyte;
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Age: adult
Comments:Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days