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平臺(tái)編號(hào)：bio-68515
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國(guó)際編號(hào)：CRL-2417?
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細(xì)胞信息： MYA-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
MYA-1
細(xì)胞類型：T淋巴細(xì)胞
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：貓
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
年限：5 months
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
器官來源：外周血
ATCC Number：CRL-2417?
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.2ml Designations: MYA-1
Depositors: ?T Miyazawa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Felis catus deposited as cat
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: pathogen-free
Cell Type: T lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 2 (IL-2)
Antigen Expression:feline CD3 +; feline CD4 +; feline CD8 -
Age: 5 months
Comments:MYA-1 is a T lymphoblastoid cell line that was established from blood mononuclear cells from a specific pathogen-free cat in 1988.
The cells are interleukin-2 (Il-2) dependent.
They are positive for IL-2 receptor, feline CD3, feline CD4 and major histocompatibility complex (MHC) class II antigen. They are negative for feline CD8.
FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells. [39933 ]
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 units/ml recombinant human IL-2 and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 39933: Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760
39934: Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：貓
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
年限：5 months
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
器官來源：外周血
ATCC Number：CRL-2417?
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.2ml Designations: MYA-1
Depositors: ?T Miyazawa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Felis catus deposited as cat
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: pathogen-free
Cell Type: T lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 2 (IL-2)
Antigen Expression:feline CD3 +; feline CD4 +; feline CD8 -
Age: 5 months
Comments:MYA-1 is a T lymphoblastoid cell line that was established from blood mononuclear cells from a specific pathogen-free cat in 1988.
The cells are interleukin-2 (Il-2) dependent.
They are positive for IL-2 receptor, feline CD3, feline CD4 and major histocompatibility complex (MHC) class II antigen. They are negative for feline CD8.
FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells. [39933 ]
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 units/ml recombinant human IL-2 and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 39933: Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760
39934: Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703
