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生長(zhǎng)狀態(tài)：貼壁，多細(xì)胞聚集
ATCC Number：CCL-147?
相關(guān)疾?。荷窠?jīng)母細(xì)胞瘤
器官來(lái)源：大腦
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
數(shù)量：大量
細(xì)胞形態(tài)：其他
規(guī)格：1ml Designations: NB41A3
Depositors: ?G Augusti-Tocco
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:loosely adherent, multicell aggregates
Organism: Mus musculus
Morphology:neuroblast; with elongated processes which emanate


Source: Organ: brain
Disease: neuroblastoma
Cell Type: neuroblast;
Cellular Products:choline acetylase; tyrosine hydroxylase; acetylcholinesterase
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Comments:Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682
26010: Burn JH, Rand MJ. Acetylcholine in adrenergic transmission. Annu. Rev. Pharmacol. 5: 163-182, 1965. PubMed: 14290806