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平臺編號：bio-68504
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國際編號：CRL-2207?
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細胞信息： PA317 LXSN 6E7
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
PA317 LXSN 6E7
運輸方式：凍存運輸
年限：embryo
器官來源：胚胎
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
細胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
品系：NIH/Swiss
ATCC Number：CRL-2207?
規(guī)格：0.2ml Designations: PA317 LXSN 6E7
Depositors: ?DA Galloway
Biosafety Level:2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:PA317 LXSN 6E7 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE7 into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
The pLXSN6bE7 vector contains the human papilloma virus (HPV) type 6b E7 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line produces the amphotropic retrovirus LXSN6bE7 which encodes the HPV6b E7 open reading frame, and which can be used to stably infect many cell types.
The resulting cells constitutively express the E7 protein of HPV6b.
The HPV6b E7 protein binds weakly to the retinoblastoma (Rb) gene.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902
年限：embryo
器官來源：胚胎
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
細胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
品系：NIH/Swiss
ATCC Number：CRL-2207?
規(guī)格：0.2ml Designations: PA317 LXSN 6E7
Depositors: ?DA Galloway
Biosafety Level:2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:PA317 LXSN 6E7 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE7 into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
The pLXSN6bE7 vector contains the human papilloma virus (HPV) type 6b E7 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line produces the amphotropic retrovirus LXSN6bE7 which encodes the HPV6b E7 open reading frame, and which can be used to stably infect many cell types.
The resulting cells constitutively express the E7 protein of HPV6b.
The HPV6b E7 protein binds weakly to the retinoblastoma (Rb) gene.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902
