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運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。阂葝u細(xì)胞瘤
ATCC Number：CRL-2055?
物種來(lái)源：小鼠
是否是腫瘤細(xì)胞：0
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
年限：10 weeks
數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
器官來(lái)源：胰腺
組織來(lái)源：islet of Langerhans
品系：NOD/Lt
規(guī)格：48T Designations: NIT-1
Depositors: ?EH Leiter
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus , transgenic for SV40 large T antigen deposited as mouse, transgenic for SV40 large T antigen
Morphology:epithelial


Source: Organ: pancreas
Strain: NOD/Lt
Tissue: islet of Langerhans
Disease: insulinoma
Cell Type: beta cell;
Cellular Products:insulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Antigen Expression:H-2 g7
Age: 10 weeks
Gender: female
Comments:The NIT-1 cell line was derived from NOD/Lt mice.
These mice are transgenic for the SV40 large T antigen under the control of a rat insulin promoter, and spontaneously develop beta adenomas.
At passage 18, most cells stained positively for insulin, less than 5% were positive for glucagon and none were positive for somatostatin or pancreatic polypeptide.
Insulin secretion is responsive to glucose concentration in the medium.
There is low constitutive expression of MHC class I, class II and ICAM-1 mRNA, but expression of both is markedly increased by treatment with interferon gamma.
Stimulation of class I mRNA is accompanied by increased class I antigen expression and induction of an occult class I product expressing the H-2.39 specificity.
MHC class II antigen is not induced despite the induction of the mRNA.
NIT-1 cells show ultrastructural features of differentiated mouse beta cells (well developed rough endoplasmic reticulum, extensive golgi apparatus and beta granules).
The cells shed a mature ecotropic type C retrovirus.
The retrovirus is capable of infecting other Fv-1 n mouse cell lines, so care should be taken to avoid cross infection.
NOTE: NIT-1 cells will not form a confluent monolayer; however, they will form nice colonies of monolayered cells in a fairly dense array.
When the NIT-1 colonies begin to ball up slightly and show many round cells on top of the monolayers as well as floating in the media, it is time to passage them.
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; heat-inactivated dialyzed fetal bovine serum, 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 1 to 2 times per week
Subcultures are prepared using a cell dissociation buffer (an enzyme free Hanks' based solution; Catalog number: 13150-016 available from GIBCO).
Remove the medium from the culture flask, add 2 ml of cell dissociation buffer per 25 sq. cm flask (5 ml per 75 sq. cm. flask and gently rock the flask at room temperature for 1 to 2 minutes to bathe the cells in the buffer.
Aspirate the solution and discard. Allow the flask to sit at room temperature for 3 to 4 additional minutes (total time from initial addition of cell dissociation buffer is approximately 5 minutes).
Firmly tap the flask against the palm of the hand to dislodge cells.
Add 5 ml of fresh medium per 25 sq. cm. flask (10 ml per 75 sq. cm. flask) and triturate up and down directing the stream along the bottom of the flask to dislodge the cells and break up some of the clumps.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
References: 22641: Hamaguchi K, et al. NIT-1, a pancreatic beta-cell line established from a transgenic NOD/Lt mouse. Diabetes 40: 842-849, 1991. PubMed: 1647994