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平臺編號：bio-68494
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國際編號：HB-10164?
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細胞信息： IB4
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
IB4
數(shù)量：大量
生長狀態(tài)：懸浮生長
是否是腫瘤細胞：0
物種來源：小鼠
運輸方式：凍存運輸
細胞形態(tài)：淋巴樣
免疫類型：IgG2a kappa
ATCC Number：HB-10164?
規(guī)格：48T Designations: IB4
Depositors: ?Rockefeller Univ.
Isotype: IgG2a kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human CD18 (leukocyte antigen)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood monocytes.
Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.
The antibody is specific for human CD18, and inhibits CD18 dependent activities.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 1194: Van Voorhis WC, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J. Exp. Med. 158: 126-145, 1983. PubMed: 6190974
22003: Wright SD, Tuomanen E. Method of inhibiting the influx of leukocytes into organs during sepsis or other trauma. US Patent 5,147,637 dated Sep 15 1992
23282: Wright SD, et al. Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies. Proc. Natl. Acad. Sci. USA 80: 5699-5703, 1983. PubMed: 6225125
生長狀態(tài)：懸浮生長
是否是腫瘤細胞：0
物種來源：小鼠
運輸方式：凍存運輸
細胞形態(tài)：淋巴樣
免疫類型：IgG2a kappa
ATCC Number：HB-10164?
規(guī)格：48T Designations: IB4
Depositors: ?Rockefeller Univ.
Isotype: IgG2a kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human CD18 (leukocyte antigen)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood monocytes.
Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.
The antibody is specific for human CD18, and inhibits CD18 dependent activities.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 1194: Van Voorhis WC, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J. Exp. Med. 158: 126-145, 1983. PubMed: 6190974
22003: Wright SD, Tuomanen E. Method of inhibiting the influx of leukocytes into organs during sepsis or other trauma. US Patent 5,147,637 dated Sep 15 1992
23282: Wright SD, et al. Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies. Proc. Natl. Acad. Sci. USA 80: 5699-5703, 1983. PubMed: 6225125
