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平臺編號：bio-68489
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國際編號：CRL-2014?
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細(xì)胞信息： HGF-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
HGF-1
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：人
年限：28 years
ATCC Number：CRL-2014?
相關(guān)疾病：正常
組織來源：gingiva
細(xì)胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
數(shù)量：大量
規(guī)格：48T Designations: HGF-1
Depositors: ?BS McAllister
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Tissue: gingiva
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: October, 1989
Applications:Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.
Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells.
Receptors:bradykinin, expressed
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,13
D5S818: 12
D7S820: 10
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Age: 28 years
Gender: male
Ethnicity: Caucasian
Comments:Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.
Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells. Bradykinin treatment blocks the proliferation stimulation by EGF and PDGF.
The cells were deposited to ATCC at an unknown passage level. A longevity study performed by ATCC showed that the depositor?s material was capable of growing for an additional 22 population doublings (i.e., unknown + 22) before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23126: McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736
23527: McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328
是否是腫瘤細(xì)胞：0
物種來源：人
年限：28 years
ATCC Number：CRL-2014?
相關(guān)疾病：正常
組織來源：gingiva
細(xì)胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
數(shù)量：大量
規(guī)格：48T Designations: HGF-1
Depositors: ?BS McAllister
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Tissue: gingiva
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: October, 1989
Applications:Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.
Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells.
Receptors:bradykinin, expressed
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,13
D5S818: 12
D7S820: 10
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Age: 28 years
Gender: male
Ethnicity: Caucasian
Comments:Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.
Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells. Bradykinin treatment blocks the proliferation stimulation by EGF and PDGF.
The cells were deposited to ATCC at an unknown passage level. A longevity study performed by ATCC showed that the depositor?s material was capable of growing for an additional 22 population doublings (i.e., unknown + 22) before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23126: McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736
23527: McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328
