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平臺編號：bio-68478
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國際編號：CRL-2280?
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細胞信息： SVR (SVEN 1 ras)
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
SVR (SVEN 1 ras)
細胞類型：SV40轉(zhuǎn)化細胞
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
器官來源：胰腺
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2280?
品系：C57BL/6
組織來源：islet of Langerhans; endothelium
運輸方式：凍存運輸
規(guī)格：30ml Designations: SVR (SVEN 1 ras)
Depositors: ?JL Arbiser
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Organ: pancreas
Strain: C57BL/6
Tissue: islet of Langerhans; endothelium
Cell Type: SV40 transformed
Cellular Products:tissue inhibitor of bioreactive matrix metalloproteinase (high levels)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:vascular endothelial growth factor (VEGF)
Tumorigenic:Yes
Comments:SVR is a derivative of MS1, a pancreatic islet endothelial cell line (see ATCC CRL-2279 ).
MS1 cells were transduced with a retrovirus encoding H-ras and hygromycin resistance.
The tumors in nude mice cause death by hemorrhage and anemia with thrombocytopenia, and can be used to test angiogenesis inhibitors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347
61244: Tsou R, et al. Retroviral delivery of dominant-negative vascular endothelial growth factor receptor type 2 to murine wounds inhibits wound angiogenesis. Wound Repair Regen. 10: 222-229, 2002. PubMed: 12191004
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
器官來源：胰腺
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2280?
品系：C57BL/6
組織來源：islet of Langerhans; endothelium
運輸方式：凍存運輸
規(guī)格：30ml Designations: SVR (SVEN 1 ras)
Depositors: ?JL Arbiser
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Organ: pancreas
Strain: C57BL/6
Tissue: islet of Langerhans; endothelium
Cell Type: SV40 transformed
Cellular Products:tissue inhibitor of bioreactive matrix metalloproteinase (high levels)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:vascular endothelial growth factor (VEGF)
Tumorigenic:Yes
Comments:SVR is a derivative of MS1, a pancreatic islet endothelial cell line (see ATCC CRL-2279 ).
MS1 cells were transduced with a retrovirus encoding H-ras and hygromycin resistance.
The tumors in nude mice cause death by hemorrhage and anemia with thrombocytopenia, and can be used to test angiogenesis inhibitors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347
61244: Tsou R, et al. Retroviral delivery of dominant-negative vascular endothelial growth factor receptor type 2 to murine wounds inhibits wound angiogenesis. Wound Repair Regen. 10: 222-229, 2002. PubMed: 12191004
