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是否是腫瘤細(xì)胞：0
物種來(lái)源：狗
年限：adult
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：其他
ATCC Number：CRL-2935?
細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：0.1ml Designations: MDCK.1
Depositors: ?Y. Reid, E. Cedrone and E-Eckard-Amar, ATCC
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Canis familiaris
Morphology:fibroblast-like


Source: Organ: kidney; distal tubule
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: January 2007
Applications:This cell line is insensitive to epsilon toxin from C.perfringens and is a control for MDCK.2 (CRL-2936)
Antigen Expression:E-cadherin (epithelial cell adhesion molecule), expressed
Zona Occludens (ZO-1) (tight junction protein), not expressed
CD29, expressed
CD18, not expressed
fibroblast-specific protein (FSP), not expressed
cytokeratin (CK1, 4, 5, 6, 8, 10, 13, 18, 19),expressed
Cytogenetic Analysis:hyperdiploid canine cell line with a modal chromosome number of 76 with low polyploidy rate. Several unidentifiable marker chromosomes were present in most of the cells examined.
Age: adult
Comments:Cell line was derived by cloning (limited dilution) the parental cell line MDCK (CCL-34). This cell line is insensitive to epsilon toxin from C.perfringens and is a control for MDCK.2 (CRL-2936)
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 X 10(4) to 3 X 10(4) viable cells/sq. cm is recommended.
6. Incubate cultures at 37C. Subculture when the cell concentration is between 8 X 10(4) and 2 X 10(5) cells/sq. cm.
   Subcultivation ratio: A subcultivation ratio of 1: 3 to 1:8 is recommended.
   Medium renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
liquid nitrogen vapor phase
Doubling Time: approximately 14 hours
Related Products:Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-30-2003
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Phosphate-buffered saline: ATCC 30-2200
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
parental cell line - ATCC CCL-34
derived from same individual - ATCC CRL-2936