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平臺編號：bio-68470
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國際編號：TIB-218?
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細(xì)胞信息： M18/2.a.12.7
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
M18/2.a.12.7 (new clone of M18/2.a.8)
ATCC Number：TIB-218?
器官來源：脾
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：rat IgG2a kappa
規(guī)格：0.1ml Designations: M18/2.a.12.7 (new clone of M18/2.a.8)
Depositors: ?TA Springer
Isotype: rat IgG2a kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the beta subunit of LFA-1 and Mac-1 (CD18)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [993 ]
Comments:Animals were immunized with mouse cytotoxic T cell glycoproteins.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The Mac-1 antigen is a macrophage differentiation antigen associated with type three complement receptor (CR3).
The LFA-1 antigen is associated with antigen specific T lymphocyte mediated cell killing.
The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.
The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen.
Tested and found negative for ectromelia virus (mousepox).
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 993: Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226
器官來源：脾
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：rat IgG2a kappa
規(guī)格：0.1ml Designations: M18/2.a.12.7 (new clone of M18/2.a.8)
Depositors: ?TA Springer
Isotype: rat IgG2a kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the beta subunit of LFA-1 and Mac-1 (CD18)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [993 ]
Comments:Animals were immunized with mouse cytotoxic T cell glycoproteins.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The Mac-1 antigen is a macrophage differentiation antigen associated with type three complement receptor (CR3).
The LFA-1 antigen is associated with antigen specific T lymphocyte mediated cell killing.
The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.
The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen.
Tested and found negative for ectromelia virus (mousepox).
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 993: Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226
