生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-1567?
組織來(lái)源：peripheral blood; chronic myelogenous leukemia
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
年限：3 years
規(guī)格：1mg Designations: NALM-1
Depositors: ?J Minowada
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: peripheral blood; chronic myelogenous leukemia
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen.
Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line.
The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,15
D5S818: 11
THO1: 7,9
TPOX: 9,11
vWA: 15,17
Cytogenetic Analysis:Contains Philadelphia chromosome (Ph1) [ Pubmed: 302341, 15588857].
Age: 3 years
Gender: female
Comments:A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia. By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen. The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed. Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line. [Pubmed: 301573].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1-2 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Preservation: Freeze medium: complete growth medium 95%,DMSO 5%
Storage temperature: liquid nitrogen vapor phase
References: 16172887: Minawada J, et al. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J. Natl. Cancer Inst. 59(1): 83-87, 1977. PubMed: 301573.
16172888: Sonta SI, et al. Cytogenetic study of a new Ph1-positive cell line (NALM-1). J. Natl. Cancer Inst. 59(3): 833-837, 1977. PubMed: 302341.
16172889: Pelz AF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet. Cytogenet. 156(1): 59-61, 2005. PubMed: 15588857.
