生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
ATCC Number：CRL-1567?
組織來源：peripheral blood; chronic myelogenous leukemia
數(shù)量：大量
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：人
年限：3 years
規(guī)格：1mg Designations: NALM-1
Depositors: ?J Minowada
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: peripheral blood; chronic myelogenous leukemia
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Applications:By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen.
Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line.
The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,15
D5S818: 11
THO1: 7,9
TPOX: 9,11
vWA: 15,17
Cytogenetic Analysis:Contains Philadelphia chromosome (Ph1) [ Pubmed: 302341, 15588857].
Age: 3 years
Gender: female
Comments:A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia. By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen. The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed. Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line. [Pubmed: 301573].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1-2 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Preservation: Freeze medium: complete growth medium 95%,DMSO 5%
Storage temperature: liquid nitrogen vapor phase
References: 16172887: Minawada J, et al. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J. Natl. Cancer Inst. 59(1): 83-87, 1977. PubMed: 301573.
16172888: Sonta SI, et al. Cytogenetic study of a new Ph1-positive cell line (NALM-1). J. Natl. Cancer Inst. 59(3): 833-837, 1977. PubMed: 302341.
16172889: Pelz AF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet. Cytogenet. 156(1): 59-61, 2005. PubMed: 15588857.
