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品系：C57BL/6
組織來(lái)源：cerebellum
年限：8 days
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：神經(jīng)元
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：CRL-2535?
器官來(lái)源：大腦
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
規(guī)格：0.1ml Designations: C8-S [Astrocyte type II clone]
Depositors: ?B Pessac, D Trisler
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:neuronal
Source: Strain: C57BL/6
Organ: brain
Tissue: cerebellum
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:pseudodiploid [48963 ]
Age: 8 days
Comments:Clonal permanent cell lines with astrocytic or microglial properties have been established from explant cultures of 8-day postnatal mouse cerebella after in vitro spontaneous transformation. [48963 ] [49701 ]
Sister flasks of lethally irradiated astrocytes, which are known to synthesize the macrophage-microglia growth factor, M-CSF, were used as a substrate for cloning the cells. [49701 ] [48963 ]
The cell lines were derived in a multistage process. Slowly proliferating foci with several morphologies appeared 4 months after initiation of the cultures and became progressively enriched by cells with a homogeneous appearance. [48963 ]
Some of these cloned cell lines bound anti-glial fibrillary acidic protein (GFAP) antibodies and therefore appeared to be astrocytic. No other glial neuronal or microglial markers have been detected in these clones. [48963 ]
According to their morphology, 3 separate types of these GFAP-positive clones could be distinguished. Type I and II cells had small somata. [48963 ]
Type I had several short processes, while type II had two processes, one of which was very thin and long (greater than 200 microns). Type III cells had large flat somata and no processes. [48963 ]
The astrocyte type I cloned cell line named C8-D1A is available as ATCC CRL-2541 and the astrocyte type III cloned cell line named C8-D30 is available as ATCC CRL-2534.
One clone with microglial properties named C8-B4 is available as ATCC CRL-2540.
The C8-S cell line has the morphology of Golgi Bergman radial cells.
These astrocytic clones might be the in vitro counterparts of fibrous (type I), or velamentous (type III) astrocytes and of Golgi epithelial cells (type II). [48963 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48963: Alliot F, Pessac B. Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella. Brain Res. 306: 283-291, 1984. PubMed: 6466977
49701: Alliot F, et al. A spontaneously immortalized mouse microglial cell line expressing CD4. Brain Res. Dev. Brain Res. 95: 140-143, 1996. PubMed: 8873987