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平臺(tái)編號(hào)：bio-68432
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國(guó)際編號(hào)：CRL-2460?
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細(xì)胞信息： MS1 VEGF
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
MS1 VEGF
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：內(nèi)皮樣
品系：C57BL/6
組織來(lái)源：islet of Langerhans; endothelium
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
數(shù)量：大量
ATCC Number：CRL-2460?
器官來(lái)源：胰腺
規(guī)格：0.2ml Designations: MS1 VEGF
Depositors: ?JL Arbiser
Biosafety Level:2 [Cells contain polyomavirus DNA sequences (SV40 large T antigen) ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:endothelial
Source: Organ: pancreas
Strain: C57BL/6
Tissue: islet of Langerhans; endothelium
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:MS1 VEGF was derived from MS1 cells (CRL-2279) by retroviral infection of the cells with a vector encoding primate vascular endothelial growth factor (VEGF).
MS1 cells were derived from primary islet endothelial cells that were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) and screened for resistance to G418.
These cells demonstrate that VEGF can transform cells in vivo.
They give rise to slow growing angiosarcomas in vivo and they demonstrate an autocrine loop between VEGF and VEGFR2.
The cells can be used to investigate signal transduction pathways involved in tumorigenesis and angiogenesis.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：內(nèi)皮樣
品系：C57BL/6
組織來(lái)源：islet of Langerhans; endothelium
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
數(shù)量：大量
ATCC Number：CRL-2460?
器官來(lái)源：胰腺
規(guī)格：0.2ml Designations: MS1 VEGF
Depositors: ?JL Arbiser
Biosafety Level:2 [Cells contain polyomavirus DNA sequences (SV40 large T antigen) ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:endothelial
Source: Organ: pancreas
Strain: C57BL/6
Tissue: islet of Langerhans; endothelium
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:MS1 VEGF was derived from MS1 cells (CRL-2279) by retroviral infection of the cells with a vector encoding primate vascular endothelial growth factor (VEGF).
MS1 cells were derived from primary islet endothelial cells that were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) and screened for resistance to G418.
These cells demonstrate that VEGF can transform cells in vivo.
They give rise to slow growing angiosarcomas in vivo and they demonstrate an autocrine loop between VEGF and VEGFR2.
The cells can be used to investigate signal transduction pathways involved in tumorigenesis and angiogenesis.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347
