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平臺編號：bio-68429
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國際編號：CRL-2133?
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細胞信息： DSL-6B/C2
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
DSL-6B/C2
數(shù)量：大量
細胞形態(tài)：上皮樣
運輸方式：凍存運輸
器官來源：胰腺
生長狀態(tài)：貼壁生長
品系：Lewis
細胞類型：其他細胞類型
ATCC Number：CRL-2133?
相關疾病：其他疾病
是否是腫瘤細胞：0
物種來源：大鼠
規(guī)格：0.5mg Designations: DSL-6B/C2
Depositors: ?OS Pettengill, D Longnecker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: pancreas
Strain: Lewis
Disease: pancreatic carcinoma
Cell Type: azaserine induced
Cellular Products:mucin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Gender: male
Comments:DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23188: Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218
細胞形態(tài)：上皮樣
運輸方式：凍存運輸
器官來源：胰腺
生長狀態(tài)：貼壁生長
品系：Lewis
細胞類型：其他細胞類型
ATCC Number：CRL-2133?
相關疾病：其他疾病
是否是腫瘤細胞：0
物種來源：大鼠
規(guī)格：0.5mg Designations: DSL-6B/C2
Depositors: ?OS Pettengill, D Longnecker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: pancreas
Strain: Lewis
Disease: pancreatic carcinoma
Cell Type: azaserine induced
Cellular Products:mucin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Gender: male
Comments:DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23188: Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218
