細(xì)胞類型：其他細(xì)胞類型
品系：C57BL/6
是否是腫瘤細(xì)胞：0
物種來源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
器官來源：睪丸
數(shù)量：大量
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2065?
相關(guān)疾?。浩渌膊?br />
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.2ml Designations: MLTC-1
Depositors: ?RV Rebois
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent, Cells form islands that dome and the monolayer may not become confluent.
Organism: Mus musculus
Morphology:epithelial
Source: Organ: testis
Strain: C57BL/6
Disease: Leydig cell tumor
Cell Type: Leydig cell;
Cellular Products:progesterone [22276 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Receptors:chorionic gonadotropin; luteinizing hormone (LH) [22276 ]
Tumorigenic:Yes
Cytogenetic Analysis:polyploid; modal number = 95 [22276 ]
Gender: male
Comments:This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice. [22276 ]
The cells retain the hormonal responsiveness of the original tumor.
Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5'-ylimidodiphosphate, and produce progesterone in response to hCG. [22276 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 35 to 40 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22276: Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
