-
平臺編號：bio-68423
-
國際編號：CCL-241?
-
細胞信息： FHs 74 Int
-
規(guī)格：frozen
-
用途：ATCC原裝細胞
-
服務費用：
加載中…… - 訂購
-
注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
FHs 74 Int
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：人
年限：3 to 4 months gestation
器官來源：小腸
數(shù)量：大量
ATCC Number：CCL-241?
相關疾?。赫?br /> 生長狀態(tài)：貼壁生長
細胞形態(tài)：上皮樣
規(guī)格：48T Designations: FHs 74 Int
Depositors: ?R Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: small intestine
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF), expressed
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Cytogenetic Analysis:normal female; diploid
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 1
PGM3, 1
Age: 3 to 4 months gestation
Gender: female
Comments:PAS, negative.
The cells are negative for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10%
(Do not filter the medium after addition of EGF)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
26058: Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205
是否是腫瘤細胞：0
物種來源：人
年限：3 to 4 months gestation
器官來源：小腸
數(shù)量：大量
ATCC Number：CCL-241?
相關疾?。赫?br /> 生長狀態(tài)：貼壁生長
細胞形態(tài)：上皮樣
規(guī)格：48T Designations: FHs 74 Int
Depositors: ?R Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: small intestine
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF), expressed
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Cytogenetic Analysis:normal female; diploid
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 1
PGM3, 1
Age: 3 to 4 months gestation
Gender: female
Comments:PAS, negative.
The cells are negative for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10%
(Do not filter the medium after addition of EGF)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
26058: Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205
